Shen LP, Chen SY, Bian T, Yi Y, Wang F, Qiu F, Bi SL
[Express the recombinant protein PD-L1 in prokaryotic and analyze its biological activity] [English Abstract, Journal Article]
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2009 Feb; 23(1):5-7.
OBJECTIVE: To construct the programmed cell death 1 ligant 1 (PD-L1) recombination expression vector, express the fusion protein in prokaryotic and analyze the biological action of express product.
METHODS: The whole PD-L1 gene sequence was synthesized after codon optimized. Construct the thioredoxin-(PD-L1) recombination expression vector and express the fusion protein in E. coli. Purified the target protein and analyze the conjugated ability of protein by ELISA.
RESULTS: The PD-L1 recombinant expression vector has been constructed correctly. The target protein has been obtained with which expressed in high efficiency and production. The target protein can conjugate specifically with the PD-1, its specific receptor.
CONCLUSION: We have obtained the PD-L1 recombinant protein success with high biological activity. The result provide the basic condition for further study on antibody and mutually action between PD-L1 and chronic virus infectious.
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